油梨不同部位总酚含量、抗氧化及抗菌活性

Total Phenolic Content, Antioxidant and Antimicrobial Activities from Different Parts of Avocado
Persea americana  Mill.


黄思思,宁德生,夏梦雯,程玲,潘争红
HUANG Sisi,NING Desheng,XIA Mengwen,CHENG Ling,PAN Zhenghong


(1.广西植物研究所,广西植物功能物质研究与利用重点实验室,广西桂林541006;2.云南师范大学化学化工学院,云南昆明650500)
(1.Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization,Guangxi Institute of Botany,Guilin,Guangxi,541006,China;2.Faculty of Chemistry and Chemical Engineering of Yunnan Normal University,Kunming,Yunnan,650500,China)


摘要:【目的】研究油梨(Persea americana Mill.)不同部位(果皮、果肉、果核、叶子)50%(V/V)甲醇提取物的总酚含量、抗氧化及抗菌活性,并分析总酚含量和抗氧化活性间的相关性。【方法】采用福林酚(Folin-Ciocaltea)法测定总酚含量,DPPH·(1,1-二苯基-2-苦肼基)自由基清除法测定抗氧化活性,SPSS 19.0双变量相关分析法分析总酚含量与抗氧化活性间的相关性,纸片法测定抗菌活性。【结果】油梨果皮的总酚含量最高,果核和叶子次之,果肉的最低。果皮、果核和叶子均具有较强的清除DPPH·自由基的能力,果肉的则相对较弱。4个部位清除DPPH·自由基能力与其所含的总酚含量存在显著的正相关关系,相关系数r值达0.983,即抗氧化能力随总酚含量的增加而增强。油梨皮和核部位对金黄色葡萄球菌(Staphylococcus aureus)有抗菌作用,但对白色念珠菌(Candida albicans)没有抑菌作用。【结论】油梨具有较强的抗氧化活性和一定的抗菌活性,为更好地综合开发和利用油梨提供理论依据。
关键词:油梨   总酚  抗氧化  抗菌
中图分类号:TS255.1      文献标识:A        文章编号:1002-7378(2016)02-0151-05
Abstract:【Objective】The aim was to investigate 50% methanol extracts of different parts(peel,pulp and seed,leaf) from avocado (Persea americana Mill.) for total phenolic content, in vitro antioxidant activity,and antimicrobial activities.The correlation between antioxidant activity and total phenolic content was also analyzed.【Methods】The contents of total phenol were measured by FolinCiocalteau assay.In vitro antioxidant activity was evaluated using the DPPH·assay.Correlation between antioxidant activity and total phenolic content was analyzed by using the Bivariate correlation analysis of SPSS 19.0 version.Antimicrobial activities were performed by disk diffusion.【Results】Peel had a higher phenolic content than seed and leaf,whereas the pulp’s was the lowest.Peel,seed and leaf had more intense in vitro antioxidant potential than the pulp. The positive correlation between the antioxidant capacity and total phenolic content was significantly (P<0.05),which  correlation coefficient was up to 0.983.In other words,the antioxidant capacity was enhanced along with the increase of total phenolic content.The peel and the seed displayed moderate antimicrobial effects against Staphylococcus aureus,but had no inhibitory effects against Candida albicans.【Conclusion】The avocado shows great intense in antioxidant potential and moderate antimicrobial effects,which provide a scientific basis to the research about comprehensive development and utilization of the avocado.
Key words:avocado (Persea americana Mill.),total phenols,antioxidant activity,antimicrobial activity

 

*广西自然科学基金青年科学基金项目(2013GXNSFBA019113)资助。

 

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发布日期:2016/6/23