濒危植物显脉金花茶的扦插繁殖试验*
Cutting Propagation in the Endangered Plant
Camellia euphlebia

 

柴胜丰,邓耘,吴儒华,何志红,刘思,宁世江,韦记青
CHAI Shengfeng,DENG Yun,WU Ruhua,HE Zhihong,LIU Si,NING Shijiang,WEI Jiqing

 

(1.广西壮族自治区中国科学院广西植物研究所,广西桂林   541006;2.柳州市园林科学研究所,广西柳州 545005;广西防城金花茶国家级自然保护区管理处,广西防城港  538021)
(1.Guangxi Institute of Botany,Guangxi Zhuangzu Autonomous Region and the Chinese Academy of Sciences,Guilin,Guangxi,541006,China;2.Liuzhou Institute of Gardening, Liuzhou,Guangxi,545005,China;3.Fangcheng Golden Camellia National Nature Reserve Management Office,Fangchenggang,Guangxi,538021,China)

 

摘要:【目的】研究显脉金花茶(Camellia euphlebia)扦插生根的最佳试验条件,为该物种的保护及规模化种植提供技术支持。【方法】采用四因素三水平正交试验设计,以生根率、生根数和平均根长为扦插生根效果的评价指标,并构建生根效果指数,研究激素种类、激素浓度、插条浸泡时间和扦插基质4个因素对显脉金花茶扦插生根的影响。【结果】扦插基质对生根效果指数的影响最大,插条浸泡时间次之,激素浓度的影响最小,只有扦插基质对生根效果指数的影响达到显著水平(P<0.05),其余3个因素的影响均未达到显著水平(P>0.05)。当激素种类为萘乙酸1-Naphthaleneacetic acid (NAA)、激素浓度为300 mg·L-1,浸泡时间为12 h,扦插基质为泥炭土+珍珠岩时,显脉金花茶扦插的生根效果最好。【结论】采用扦插繁殖技术开展显脉金花茶的大规模育苗是可行的。
关键词:显脉金花茶  濒危植物   扦插繁殖   正交试验
中图分类号:Q945.5         文献标识码:A           文章编号:1002-7378(2016)01-0015-06
Abstract:【Objective】In order to provide technical support for protection and large-scale planting of Camellia euphlebia,the experiment was designed to find out the optimum conditions of cuttings rooting in this species.【Methods】By using 1 or 2-year-old branches of C.euphlebia,and taking rooting rate,rooting number,root length,and rooting effect index as evaluation index,the effect of four factors including hormone types and concentration,treating time,substrate types on rooting of cuttings with orthogonal design L9(34) were studied.【Results】The substrate types were the key factor affecting rooting of cuttings. Treatment time was second effect on rooting of cuttings. Hormone concentration was least effect on rooting of cuttings.The substrate types had significant effect on rooting effect index of cuttings,while other 3 factors didn’t show any significant effect. The optimum treatment condition is to treat cuttings with 300 mg·L-1 NAA for 12 h and taking peat soil + perlite as substrate.【Concusion】The cutting propagation is feasible to carry out large-scale breeding of C.euphlebia.
Key words:Camellia euphlebia,endangered plant,cutting propagation,orthogonal design

 

*国家自然科学基金项目(31160137),广西科技成果转化与推广计划项目(桂科转14125003-2-18),广西科学研究与技术开发计划项目(桂科重1355001-5-4),桂林市科技攻关项目(20130414)和广西自然科学基金项目(2013GXNSFAA019081)资助。

 

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